EpiOcular Eye Irritation Test
The Model
MatTek’s EpiOcular system consists of normal, human-derived epidermal keratinocytes which have been cultured to form a stratified, squamous epithelium similar to that found in the cornea. Cultured on specially prepared cell culture inserts using serum-free culture medium, the cells differentiate to form a multi-layered structure which closely parallels the corneal epithelium. For more information on the EpiOcular tissue model, click here.
The Method
Request the full protocol here.
The Endpoints
MTT Tissue Viability Assay
Technical References
729. COSMETICS EUROPE MULTI-LABORATORY PRE-VALIDATION OF THE EPIOCULAR™ RECONSTITUTED HUMAN TISSUE TEST METHOD FOR THE PREDICTION OF EYE IRRITATION.
Pfannenbecker1, U., Bessou-Touya2, S., Faller3, C., Harbell4, J., Jacob5, T., Raabe6, H., Tailhardat7, M., Alépée8, N., De Smedt9, A., De Wever10, B., Jones11, P., Kaluzhny12, Y., Le Varlet13, B., McNamee14, P., Marrec-Fairley15, M., Van Goethem9, F. 1Beiersdorf AG, Hamburg, Germany. 2Laboratoire Pierre Fabre, Castres, France. 3Procter & Gamble/Cosmital, Marly, Switzerland. 4Mary Kay Inc., Dallas, TX, USA. 5Avon Products, Inc., Suffern, NY, USA. 6Institute for In Vitro Sciences, Inc., Gaithersburg, MD, USA. 7LVMH Recherche, St. Jean De Braye Cedex, France. 8L’Oréal Research & Innovation, Aulnay Sous Bois, France. 9Janssen Research & Development, Beerse, Belgium. 10Henkel AG & Co. KGaA, Düsseldorf, Germany. 11Safety and Environmental Assurance Centre, Unilever, Sharnbrook, UK. 12MatTek Corporation, Ashland, MA, USA. 13Consultant, Links Ingénierie, Paris, France. 14The Procter & Gamble Company, Egham, Surrey, UK. 15Colipa, Brussels, Belgium. Toxicology in Vitro, 27, 619–626, (2013).
706. PREDICTING EYE IRRITATION OF AGROCHEMICAL FORMULATIONS ACCORDING TO DIFFERENT CLASSIFICATION SCHEMES BY IN VITRO METHODS (BOVINE CORNEAL OPACITY AND PERMEABILITY AND EPIOCULAR EYE IRRITATION TEST).
Kolle1, S.N. Buechse2, A., Knieriem3, T., Mayer3, W., Rey Moreno1, M-C., van Cott4, A., van Ravenzwaay1, B., and Landsiedel1, R. 1BASF SE, Experimental Toxicology and Ecology, Ludwigshafen, Germany, 2BASF SE, Scientific Computing, Ludwigshafen, Germany, 3BASF SE, Agricultural Products – Formulation Development, Ludwigshafen, Germany, 4BASF Cooperation, Agricultural Products – Global Product Safety & Registration, Raleigh, NC, USA. EuroTox 2012.
683. UPDATE ON VALIDATION STATUS AND INDUSTRY UTILIZATION OF NORMAL HUMAN 3D (NHU-3D) ANIMAL ALTERNATIVE MODELS.
Kandarova, H., Klausner, M., Kubilus, J., Ayehunie, S., Hayden, P., Kaluzhny, Y., Letasiova, S., and Sheasgreen, J. MatTek Corporation, Ashland, MA, USA and MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia. Presented at 8th World Congress on Alternative and Animal Use, Montreal, Canada, 2011.
681. PROSPECTIVE VALIDATION STUDY OF RECONSTRUCTED HUMAN TISSUE MODELS FOR EYE IRRITATION TESTING.
McNamee1,2, P., Alépée3, N., Barroso4, J., Cole4, T., de Jong-Rubingh5, C., Eskes6, C., Freeman7, S.J., Kirmizidis4, G., Lammers8, J., Pfannenbecker9, U. and Zuang4, V. 1The Procter & Gamble Company, U.K.,2COLIPA, Belgium; 3L’Oréal, France; 4ECVAM, Institute for Health & Consumer Protection, European Commission Joint Research Centre, Italy; 5TNO, The Netherlands; 6Services & Consultation on Alternative Methods (SeCAM), Agno, Switzerland; 7Farino Consulting, UK; 8TNO Triskelion, The Netherlands; 9Beiersdorf, Germany. Presented at 8th World Congress on Alternatives and Animal Use, Montreal, Canada, 2011.
673. IN-HOUSE VALIDATION OF THE EPIOCULAR™ EYE IRRITATION TEST AND ITS COMBINATION WITH THE BOVINE CORNEAL OPACITY AND PERMEABILITY TEST FOR THE ASSESSMENT OF OCULAR IRRITATION.
Kolle1, S.N., Kandárová2, H., Wareing1, B., van Ravenzwaay1, B., and Landsiedel1, R. 1BASF SE, Experimental Toxicology and Ecology, Ludwigshafen, Germany; 2MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia. ATLA, 39, 365–387, (2011). ATLA, 39, 365–387, (2011).
653. DEVELOPMENT OF THE EPIOCULAR™ EYE IRRITATION TEST FOR HAZARD IDENTIFICATION AND LABELLING OF EYE IRRITATING CHEMICALS IN RESPONSE TO THE REQUIREMENTS OF THE EU COSMETICS DIRECTIVE AND REACH LEGISLATION.
Kaluzhny1, Y., Kandárová2, H., Hayden1, P., Kubilus1, J., d’Argembeau-Thornton1, L., and Klausner1, M. 1MatTek Corporation, Ashland, MA, USA; 2MatTek In Vitro Life Science Laboratories, Bratislava, Slovakia. ATLA, 39, 339-364, (2011).
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